Conformational Polymorphism in Conditionally Disordered Nucleophosmin: From Single-Molecules to Liquid Droplets
نویسندگان
چکیده
منابع مشابه
Conformational fluctuations in single DNA molecules.
Measurement of fluorescent lifetimes of dye-tagged DNA molecules reveal the existence of different conformations. Conformational fluctuations observed by fluorescence correlation spectroscopy give rise to a relaxation behavior that is described by "stretched" exponentials and indicates the presence of a distribution of transition rates between two conformations. Whether this is an inhomogeneous...
متن کاملsingle nucleotide polymorphism and asthma: from conformational variations to structural alternations
background: asthma is the main reason of disability, health resource exploitation and low quality of life for those who are affected. it is estimated that nearly 300 million people in the world are suffering from asthma. studies have identified 18 genomic regions and more than 100 genes associated with asthma. among these candidate genes, il-17f plays a very interesting role in asthma. this stu...
متن کاملLigand-induced conformational changes observed in single RNA molecules.
We present the first demonstration that fluorescence resonance energy transfer can be used to track the motion of a single molecule undergoing conformational changes. As a model system, the conformational changes of individual three-helix junction RNA molecules induced by the binding of ribosomal protein S15 or Mg(2+) ions were studied by changes in single-molecule fluorescence. The transition ...
متن کاملOptical vortices from liquid crystal droplets.
We report on the generation of mono- and polychromatic optical phase singularities from micron-sized birefringent droplets. This is done experimentally by using liquid crystal droplets whose three dimensional architecture of the optical axis is controlled within the bulk by surfactant agents. Because of its microscopic size these optical vortex generators are optically trapped and manipulated a...
متن کاملDisordered binding of small molecules to Aβ12-28 DISORDERED BINDING OF SMALL MOLECULES TO Aβ12-28
Background: Inhibition of pathogenic protein aggregation by small molecules is poorly understood. Results: Aggregation inhibitors alter the free energy landscape of a relevant fragment of Alzheimer's amyloid-β protein in subtle but complex fashion. Conclusion: Intrinsic disorder of disease proteins persists at the level of binding small molecules. Significance: Hallmark characteristics and effi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.2171